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KMID : 0613820110210060775
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Journal of Life Science
2011 Volume.21 No. 6 p.775 ~ p.782
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Antioxidant Capacity and Quinone Reductase Activity of Methanol Extracts and Fractions from Papaya Seed
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Yu Me-Hee
Lee Sung-Gyu
Im Hyo-Gwon
Chae In-Gyeong
Kim Hyun-jung
Lee Jin-Ho
Lee In-Seon
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Abstract
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In this study, the antioxidant activity of methanol extracts and fractions from papaya seed were investigated in vitro. Total polyphenol contents of methanol extracts and fractions from papaya seed varied from 17.74 to 125.99 ㎍/㎎ and total flavonoid contents varied from 1.60 to 32.69 ㎍/㎎. Contents of polyphenol and flavonoid in ethyl acetate (EtOAc) fraction was found to be extremely high (compared with the other fractions examined). Radical-scavenging activities of methanol extracts and fractions were examined using α,α-diphenyl-β-picrylhydrazyl (DPPH) radicals, 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and hydrogen peroxide assay. As a result, ethyl acetate fraction of papaya seed showed the highest radical-scavenging activity in various antioxidant systems. The EtOAc fraction from papaya seed induced QR activity in concentrations of 12.5 to 50 ㎍/㎖ with a maximum of a 3.3-fold induction at 50 ㎍/㎖ of fraction. Therefore, the most effective QR inducer among these fractions can be said to reside in the EtOAc fraction, indicating that strong constituents responsible for QR induction potency in the papaya seed extract are largely contained in the EtOAc fraction.
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KEYWORD
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Papaya, seed, antioxidant, quinone reductase, Hepa1c1c7 cells
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